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| Dórea,Regina Coeli Cunha; Barboza-Filho,Carlos Gomes; Moreira,Dayson Friaça; Guirro,Mirian; Chaves,Ágtha de Alencar Muniz; Dunder,Ricardo José; Soares,Elaine Amorim; Santos-Júnior,Vitório; Spinosa,Wilma. |
| Little is known on the epitopes derived from metacyclic promastigotes of Leishmania that are important on the regulation or destruction of the parasite, as targets of immune attack in the vertebrate host. In this study we investigated an alternative method to obtain metacyclic promasigotes of Leishmania major, as evaluated by the course of infection and delayed-type hipersensitivity (DTH) in resistant versus susceptible inbred mice. Non-infective (procyclic) promastigotes of L. major recently transformed from tissue amastigotes were attached to a negatively charged glass-wool column, whereas metacyclic promastigotes were not bound to columns and could be easily recovered. Optimal chromatography conditions were validated through statistical analyses.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania major; Metacyclic promastigotes; Metacyclogenesis; BALB/c mice; C57BL/6 mice. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500006 |
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| LANZA,HENRIQUE; AFONSO-CARDOSO,SANDRA R; SILVA,ADRIANO G; NAPOLITANO,DANIELLE R; ESPÍNDOLA,FOUED S; PENA,JANETHE D.O; SOUZA,MARIA A. |
| Amastigotes of Leishmania major have a great ability to evade destruction in host cells. This study investigated the activation in resident, inflammatory macrophages and J774 cells in vitro treated with lipopolysaccharide (LPS), soluble Leishmania antigen (SLA), calcium ionophore (CaI) and magnesium (Mg2+) alone or combined. An increase in nitric oxide (NO) production was observed in J774 or inflammatory macrophages treated with LPS alone or in combination with SLA and CaI. The same treatments did not affect the NO release by resident macrophages. There was no interference in uptake of L. major but CaI decreased intracellular proliferation of the parasite. This study demonstrated the importance of CaI in decreasing L. major proliferation inside murine... |
| Tipo: Journal article |
Palavras-chave: Calcium; Leishmania major; Macrophage; Magnesium; Nitric oxide. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300004 |
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| Zahirnia,Amir Hossein; Bordbar,Ali; Ebrahimi,Sahar; Spotin,Adel; Mohammadi,Somayeh; Ghafari,Seyedeh Maryam; Ahmadvand,Setareh; Jabbari,Negar; Esmaeili Rastaghi,Ahmad Reza; Parvizi,Parviz. |
| ABSTRACT Background Leishmania major is a causative agent of zoonotic cutaneous leishmaniasis in the center of Iran, Abarkouh district. Molecular characterization and precise incrimination of Leishmania species was carried out to perform controlling measurements and to design treatment programs for zoonotic cutaneous leishmaniasis. Methods All smears isolated from ulcers of suspected patients were examined under a light microscope and graded for amastigotes frequency. Extraction of DNA, PCR, RFLP and sequencing of ITS-rDNA genotype were done to increase the efficacy of Leishmania parasites identification at their species-specific level and to detect any Leishmania infections within. Results Humans were found to be infected with L. major with high... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmaniasis; Leishmania major; Leishmania tropica; Nuclear gene; Iran. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000400278 |
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| Teixeira,Santuza M.; Paiva,Rita Márcia Cardoso de; Kangussu-Marcolino,Monica M.; DaRocha,Wanderson D.. |
| In 2005, draft sequences of the genomes of Trypanosoma brucei, Trypanosoma cruzi and Leishmania major, also known as the Tri-Tryp genomes, were published. These protozoan parasites are the causative agents of three distinct insect-borne diseases, namely sleeping sickness, Chagas disease and leishmaniasis, all with a worldwide distribution. Despite the large estimated evolutionary distance among them, a conserved core of ~6,200 trypanosomatid genes was found among the Tri-Tryp genomes. Extensive analysis of these genomic sequences has greatly increased our understanding of the biology of these parasites and their host-parasite interactions. In this article, we review the recent advances in the comparative genomics of these three species. This analysis also... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypanosoma brucei; Trypanosoma cruzi; Leishmania major; Genome; RNAseq. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100001 |
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